Part:BBa_K4601000

CatCh

This part is the E. coli codon optimised version of CatCh channelrhodopsin.

Usage and Biology

CatCh is a channelrhodopsin derived from the ChR2 of Chlamydomonas reinhardtii [1], from which it only retains the first 309 amino acids containing the N-terminal transmembrane domain forming. Compared to ChR2, it also contains one mutation L132C that modifies the photocurrent generated upon light irradiation. CatCh is a Ca2+ inward pump that is also capable of transporting, although to a lesser extent, Na⁺, K⁺ and Mg²⁺. It was successfully used in optogenetic therapies for visual restoration in primates [2].

For the characterisation of this part an expression cassette was assembled and the results are presented on its page in Parts Registry: BBa_K4601200.

References

[1] Kleinlogel S, Feldbauer K, Dempski RE, Fotis H, Wood PG, Bamann C, Bamberg E. Ultra light-sensitive and fast neuronal activation with the Ca2+-permeable channelrhodopsin CatCh. Nature Neuroscience (2011) 14: 513–518.

[2] Chaffiol A, Caplette R, Jaillard C, Brazhnikova E, Desrosiers M, Dubus E, Duhamel L, Macé E, Marre O, Benoit P, Hantraye P, Bemelmans A-P, Bamberg E, Duebel J, Sahel J-A, Picaud S, Dalkara D. A new promoter allows optogenetic vision restoration with enhanced sensitivity in macaque retina. Molecular Therapy: The Journal of the American Society of Gene Therapy (2017) 25: 2546–2560.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 623
  Illegal AgeI site found at 508
  
• 1000
  COMPATIBLE WITH RFC[1000]